Development of nine polymorphic microsatellite loci in the spined loach,Cobitis taenia,and cross-species amplification in the related speciesC. elongatoides,C. tauricaandC. tanaitica

K. DE GELAS, K. JANKO, F. A. M. VOLCKAERT, D. DE CHARLEROY, J. K. J. VAN HOUDT
2008 Molecular Ecology Resources  
Nine polymorphic microsatellite loci were developed for the spined loach, Cobitis taenia (Teleostei: Cobitidae). The loci were validated using 50 individuals from a population in Belgium. Moderate to high levels of polymorphism were detected (two to 11 alleles). In addition, most markers amplified successfully in three closely related taxa that are known to hybridize with C. taenia: C. elongatoides, C. taurica and C. tanaitica. Some of the loci are most likely diagnostic among species. These
more » ... kers will be valuable for the study of the historical and contemporary interactions within C. taenia and the Cobitis species complex.
doi:10.1111/j.1755-0998.2008.02135.x pmid:21585954 fatcat:jp54d5opanhotnk4cwjztgq6lu