Recombinant Escherichia coli clones expressing Chlamydia trachomatis gene products attach to human endometrial epithelial cells

D H Schmiel, S T Knight, J E Raulston, J Choong, C H Davis, P B Wyrick
1991 Infection and Immunity  
macromolecules on the infectious EB and the host cell participate in chlamydial attachment. Exposure of EB to conditions and reagents which reduce adherence to tissue culture cells include (i) exposure to heat (60°C) for 3 min, (ii) periodate treatment, and (iii) acetic anhydride treatment (4, 14) . Adherence of chlamydiae is also reduced by exposing the tissue culture cells to periodate and trypsin; the effect of the latter is reversible over time except in the presence of cycloheximide, which
more » ... ycloheximide, which inhibits eukaryotic protein synthesis (4, 14) . These data suggest that the EB and the host cell have both surface carbohydrate and proteinaceous moieties, perhaps glycoproteins, which are involved in chlamydial attachment. Several questions remain unanswered. (i) Do the treatments mentioned above affect some global surface property, such as charge or hydrophobicity, or are certain macromolecules, i.e., a chlamydial adhesin(s) and eukaryote receptor(s), involved in specific interactions that mediate attachment? (ii) Is chlamydial adherence a one-step process or is it a multistep process in which electrostatic interactions might mediate initial attachment but are followed by more specific interactions (49)? A role for electrostatic interactions is suggested by findings that the polycation DEAE-dextran improves the infec-4001 on May 7, 2020 by guest http://iai.asm.org/ Downloaded from
doi:10.1128/iai.59.11.4001-4012.1991 fatcat:rl5nboy4nvaevcuak6icqhlkdu