Association of ACE Gene with Metabolic Syndrome, Hypertension and Alzheimer Disease by RFLP-PCR

Stanislav Alexandra Alina
2020 Journal of Chemistry and Chemical Engineering  
ACE gene is associated with multifactorial diseases: metabolic syndrome, obesity, diabetes mellitus, hypertension, stroke, myocardial infarction, ageing, Alzheimer disease, acute pulmonary failure and COVID-19 infections. The purpose of this study is to test the association between the II, ID and DD polymorphisms of angiotensin-converting enzyme (ACE) gene, and metabolic syndrome, hypertension (HBP) and Alzheimer disease (AD), based on clinical data and biochemical laboratory investigations
more » ... investigations conducted on inpatients, applying the RFLP-PCR technique. The genotyping of ACE gene was carried out by RFLP-PCR on the basis of DNA isolated from total blood in 144 subjects selected at Giurgiu County Emergency Hospital. The results were statistically processed by the Hardy-Weinberg equilibrium, Odds Ratio, VMD, StatsDirect and PyElph software. The II, ID and DD polymorphisms of ACE gene identified by the RFLP-PCR present a high risk of developing the metabolic syndrome in the MS, hypertension and Alzheimer disease groups.
doi:10.17265/1934-7375/2020.04.001 fatcat:66nmylrnong4fg4lxawbd6seme