PATTERN OF SHEATH SYNTHESIS IN SPHAEROTILUS NATANS

Antonio H. Romano, Dorothy J. Geason
1964 Journal of Bacteriology  
ABsriAt.v(Cr RO.N\ANO, ANTONI) H. (University of Ciilciiniati, Cincininati, Ohio), AND DOROvHY J. GEAsoN. Pattern of sheath synthesis in Sphaerotilu(s natans. J. Bacteriol. 88:1145Bacteriol. 88: -1150Bacteriol. 88: . 1964.-Formation of the characteristic sheath of Sphaerotiluis natans was followed by imnmunofluoreseence. Fluorescenit antisheath antibody was obtained by injecting rabbits with purified sheath material fromn S. natans, fractionatinig the serurm with amiiioinium sulfate, and
more » ... sulfate, and conjugating the globulin fractioii with fluorescein isothiocyanate. To follow sheath formnation, S. natans was grown on slides immenrsed in Stokes' medium at 28 C for 9 hr, and was reacted with labeled antibody. The slide cultures were then washed to remove unibouind antibody, and were inicubated further in fresh medium. Samples were removed at initervals and examinied by fluorescence microscopy. Old portions of the
doi:10.1128/jb.88.4.1145-1150.1964 fatcat:udmfpnairrg7dgdpd5sblh64bu