Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows

Andre Penido Oliveira, Marcos Bryan Heinemann, Adriana Cortez, Paula Maria Pires do Nascimento, Romulo Cerqueira Leite, João Henrique Moreira Viana
2016 Pesquisa Agropecuária Brasileira  
Abstract: The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid.
more » ... l DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid.
doi:10.1590/s0100-204x2016000500031 fatcat:lsuuyjlkxnbhjlhv7xzqgj3arq