The aim of this study was to examine the effect of Vibration, PNF (Proprioceptive Neuromuscular Facilitation) and mixture stretching method on flexibility of the hamstring muscles in non-athlete male students. Material and Methods: In this study, 30 students were selected through goal oriented method and then divided into 3 groups: vibration (N=10), PNF (N=10), and mixture (N=10). Firstly, the flexibility of the hamstring muscles was measured by the "passive leg straight test". Then, the 3

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... s were trained for 4 weeks. After that, passive leg straight test was performed in the three groups. Results: The results indicated that the flexibility in all three groups significantly increased after training protocol (p=0.001). Although in the hamstring muscles there was no significant difference between the flexibility of the vibration and PNF groups (P>0.05), in the mixture group flexibility was significantly more than the other groups (p=0.000). Conclusion: Based on the results of this study, although the athletes achieve relatively favorable levels by vibration or PNF methods, the combination of these methods contribute to the improvement of flexibility to the highest degree. Therefore, stretch training method is recommended in such sports as gymnastic, wrestling and dancing which need a high flexibility.