Suppression of nucleosome-binding protein 1 by miR-326 impedes cell proliferation and invasion in non-small cell lung cancer cells

DONGFAN LI, XUSHENG DU, AN LIU, PENG LI
2015 Oncology Reports  
Emerging studies have proposed microRNAs (miRNAs) as novel therapeutic tools for cancer therapy. Nucleosome-binding protein 1 (NSBP1) has been suggested as an oncogene in various types of human cancers. The present study aimed to identify a novel miRNA that could directly target and negatively modulate NSBP1 expression. We found that NSBP1 was highly expressed in non-small cell lung cancer (NSCLC) cells, and knockdown of NSBP1 by NSBP1 small interfering RNA (siRNA) significantly suppressed
more » ... tly suppressed NSCLC cell proliferation and invasion. Bioinformatics analysis revealed that miR-326 had a putative binding site within the 3'-untranslated region of NSBP1. Their substantial relationship was further verified by dual-luciferase reporter assay, real-time quantitative polymerase chain reaction and western blot analysis. Overexpression of miR-326 significantly inhibited NSCLC cell proliferation and invasion, which mimicked the effect of NSBP1 siRNA. Furthermore, suppression of NSBP1 by NSBP1 siRNA or miR-326 overexpression remarkably repressed the expression of cyclin B1 and matrix metalloproteinase 9 (MMP9), which are associated with cancer cell proliferation and invasion. Moreover, overexpression of NSBP1 obviously abolished the inhibitory effect of miR-326 on cyclin B1 and MMP9 expression. In addition, an inverse correlation between miR-326 and NSBP1 expression levels was found in NSCLC clinical specimens. Our study demonstrated a direct target relationship between NSBP1 and miR-326 through which miR-326 inhibited cell proliferation and invasion of NSCLC cells. Thus, miR-326-NSBP1 is a promising candidate target for developing novel anticancer therapeutics for NSCLC. Abbreviations: miRs, microRNAs; UTR, untranslated region; NSBP1, nucleosome-binding protein; NSCLC, non-small cell lung cancer; MMP9, matrix metalloproteinase-9
doi:10.3892/or.2015.4403 pmid:26548724 fatcat:4bfcba3yizahhbfkdl3er4dd4a