There are two methods to search the spot in the microarray DNA chip: Grid method and Circle template image method. However, these methods have the drawback that they are slow. To solve this problem in a recent study, applying the running algorithm using a box template image reduced the time by 1/10. However, there was a drop in accuracy when comparing accuracy with Grid and Circle template matching methods. Since the size of the box template image used in the previous study is the same as the

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... ot size, the circular spot and the template image do not coincide with each other. Therefore, we show the difference of accuracy by changing the size of the template image. In this paper, we measure the accuracy of the box template image by size and find the best performance. As a result, it seems to be grounds for selecting the most appropriate size box template image according to spot size.