The purpose of this study was to perform a comparative study of cations in the vegetative organs of Sambucus ebulus by applying three different methods of digestion before atomic absorption spectrometry. Of the tested methods, the most effective was method 3 (mineralisation with nitric acid in a microwave oven), the other methods of sample digestion leading to incomplete mineralization of the samples and implicitly to the incomplete transfer of the analytes into solution, reflecting the slightly different contents in metal cations we obtained.