Mesorhizobium shangrilense sp. nov., isolated from root nodules of Caragana species

Y. L. Lu, W. F. Chen, E. T. Wang, L. L. Han, X. X. Zhang, W. X. Chen, S. Z. Han
2009 International Journal of Systematic and Evolutionary Microbiology  
Five strains of bacteria isolated from nodules of Caragana bicolor and Caragana erinacea in Yunnan Province of China were classified within the genus Mesorhizobium in the class Alphaproteobacteria. The highest degree of 16S rRNA gene sequence similarity was determined to be to Mesorhizobium loti LMG 6125 T (99.7 %) and Mesorhizobium ciceri UPM-Ca7 T (99.7 %). Polyphasic taxonomic methods including SDS-PAGE of whole-cell soluble proteins, comparative housekeeping sequence analysis of atpD, glnII
more » ... and recA, fatty acid profiles and a series of phenotypic and physiological tests allowed us to cluster the five strains into a coherent group while differentiating them from all previously established Mesorhizobium species. The DNA-DNA relatedness between the representative strain CCBAU 65327 T and the type strains of M. loti and M. ciceri was 26.5 and 23.4 %, respectively, clearly indicating that strain CCBAU 65327 T represents a novel species for which we propose the name Mesorhizobium shangrilense sp. nov. Strain CCBAU 65327 T (5LMG 24762 T 5HAMBI 3050 T ) is designated as the type strain, and could nodulate Caragana microphylla, Caragana intermedia, Glycyrrhiza uralensis, Astragalus adsurgens, Vigna unguiculata, Vigna radiata and Phaseolus vulgaris in cross-nodulation tests. Abbreviations: MP, maximum-parsimony; NJ, neighbour-joining. The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA, atpD, recA, glnII, nodC and nifH gene sequences of strain CCBAU 65327 T are EU074203, EU672471, EU672501, EU672486, EU687487 and EU877532, respectively. Fatty acid profiles, sequence similarities, nodC and nifH gene phylogenetic trees and a phenogram derived from UPGMA/S SM analysis and SDS-PAGE analysis of whole-cell soluble proteins of strains from Mesorhizobium shangrilense sp. nov. and related species are available as supplementary material with the online version of this paper.
doi:10.1099/ijs.0.007393-0 pmid:19643905 fatcat:odwmuimvhrdstfygt3bntjq2se