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The essential genome of Escherichia coli K-12
[article]
2017
bioRxiv
pre-print
Transposon-Directed Insertion-site Sequencing (TraDIS) is a high-throughput method coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used to identify essential genes. Single gene deletion libraries are considered the gold standard for identifying essential genes. Currently, the TraDIS method has not been benchmarked against such libraries and therefore it remains unclear whether the two methodologies are comparable. To address this, a high density transposon
doi:10.1101/237842
fatcat:abe5fuekwzgbzksyrg6yp45kty