Ion mobility mass spectrometry: an elegant alternative focusing on speciation studies

Gustavo de Souza Pessôa, Eduardo Jorge Pilau, Fábio Cesar Gozzo, Marco Aurelio Zezzi Arruda
2011 Journal of Analytical Atomic Spectrometry  
This work is proposed to demonstrate the Traveling-Wave Ion Mobility Specrometry (TWIMS) coupled to Mass Spectrometry (MS) as an alternative technique for speciation analysis between metals/ metalloids and biomolecules. Mobilities of bovine carbonic anhydrase bound to Ba 2+ , Cu 2+ , Pb 2+ , Zn 2+ , Cr 3+ , Cr 6+ , Se 4+ and Se 6+ were estimated. The metal belonging to the bovine carbonic anhydrase structure, commonly found in the commercially available enzyme, was removed by filtration, using
more » ... filtration, using centrifugal filter devices. Then, some metals/metalloids were added to 10.0 mmol L À1 ammonium acetate at pH ¼ 6.8 enzyme solution. Experiments were carried out by direct insertion of the sample at 10 mL min À1 flow rate into the ESI source of the instrument. Carbonic anhydrase mobility varied according to the metal bound in its structure, following the order: Zn 2+ < Cu 2+ < Ba 2+ < Pb 2+ . Metals with higher affinity by the enzyme, such as Zn 2+ and Cu 2+ had lower mobility, suggesting a higher structural modification, binding itself to the enzyme metallic site. Considering metals with different oxidation states, the enzyme mobility followed the order: Se 4+ < Cr 6+ < Se 6+ < Cr 3+ .
doi:10.1039/c0ja00139b fatcat:wnlu7pwc7fbbbhbxtff6hf2gm4