Hypoxic conditioned medium of placenta-derived mesenchymal stem cells inhibits TGF-β1-induced up-regulation of collagens and differentiation of skin fibroblasts to myofibroblast through IL-10

Lili Du, Runxiao Lv, Shaohang Cheng, Xiaoyi Yang, Tingxian Ma, Jing Xu
2016 Int J Clin Exp Pathol   unpublished
Scar formation affects the quality of people's daily lives, and in this study we explored whether hypoxic conditioned medium of placenta-derived mesenchymal stem cells (PMSCs) has a protective effect on scar formation. Our study showed that hypoxic conditioned medium of PMSCs inhibited the transforming growth factor-β1 (TGF-β1)-induced up-regulation of collagens and differentiation of skin fibroblasts to myofibroblast. Mechanism study showed that the level of interleukin-10 (IL-10) was
more » ... IL-10) was influenced by hypoxic culture condition of PMSCs and further study showed that IL-10 simulated the effects of hypoxic conditioned medium of PMSCs to inhibit TGF-β1-induced up-regulation of collagens and α-smooth muscle actin (α-SMA), whereas, the inhibition effects of hypoxic conditioned medium of PMSCs on TGF-β1-induced up-regulation of collagens and α-SMA was reversed by IL-10 antibody. Results of this study suggested that hypoxic conditioned medium of PMSCs performed a protective effect against scar formation through the regulation of IL-10. This study provides further evidence for the possible use of PMSCs as a promising method to treat wounds.
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