Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, Jianan Y. Qu
2012 Optics Letters  
We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).
more » ... d cells (neutrophils).
doi:10.1364/ol.37.002490 pmid:22743431 fatcat:zqpqy6wsgvch7i4amwn2a6hvda