Simultaneous determination of Nickel (II) and Copper (II) using 4-Hydroxy-3, 5-dimethoxy benzaldehyde-4-hydroxybenzoylhydrazone by first order derivative spectrophotometric technique

D Gopala Krishna, N Devanna, Ch Kethani, Devi
2012 International Journal of Analytical and Bioanalytical Chemistry   unpublished
4-Hydroxy-3,5-dimethoxybenzaldehyde-4-hydroxybenzoylhydrazone (HDMBHBH) was used as chromogenic reagent for Simultaneous spectrophotometric determination of Nickel (II) and Copper (II) ions. The reagent (HDMBHBH) gives intense yellow colour water soluble complexes with Nickel (II) and Copper (II) in presence of micellar medium (Triton-X-100 (5%) at pH 9.0. The maximum absorbance was observed at max 408 and 383 nm for Nickel (II) and Copper (II) respectively. The molar absorptivity and sandell's
more » ... ivity and sandell's sensitivity for Nickel (II) and Copper (II) complexes are found to be 3.22 x 10 4 , 3.41x10 4 L.mol-1 .cm-1 and 0.0018, 0.0018 µg/cm 2 respectively. The reagent (HDMBHBH) forms 1:1 [M: L] complexes with Nickel (II) and Copper (II) with stability constants 5x10 6 and 14.12x10 6 respectively. The First order derivative spectrophotometric technique has been developed for the simultaneous determination of Nickel (II) and Copper (II) at max 450 and 405 nm in Synthetic mixtures, Alloy samples and Edible oils.