Hyperin and quercetin are phenolic compounds present in fruits and vegetables that have been reported to possess strong anti-oxidative properties. Although increasing evidence strongly suggests that antioxidants suppress the melanin synthesis that is causally associated with oxidative stress, the protective actions of hyperin and quercetin against oxidative stress-induced melanogenesis have not been fully explored. To elucidate the suppressive effects of hyperin and quercetin on oxidative

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... and melanin synthesis, peroxynitrite (ONOO ) scavenging activity was measured in vitro as were quantifications of melanin content, intracellular total RS, ONOO , superoxide ( • O 2 ), nitric oxide (NO • ), catalase activity and the reduced glutathione (GSH)/ oxidized glutathione (GSSG) ratio. Results showed that in vitro, hyperin and quercetin reduced ONOO . Additionally, hyperin and quercetin suppressed total RS, ONOO , • O 2 , and NO • , catalase activity, and melanin synthesis, while they boosted the GSH/GSSG ratio in B16F10 melanoma cells (B16 cells). Therefore, I propose that hyperin and quercetin have a powerful capacity to modulate oxidative stress-induced melanogenesis.