Fibroblasts attachment to CaCl2 hydrothermally treated titanium implant

W. Elshahawy, I. Watanabe, M. Nakagawa, P. Kramer
2014 Tanta Dental Journal  
Background/Purpose: This study investigated the effect of hydrothermal treatment with calcium chloride (CaCl 2 ) solutions on fibroblast attachment and proliferation to titanium implants in the presence and absence of fetal bovine serum. Materials and methods: Pure titanium specimens were prepared, polished (1500 grit), cleaned with ethanol, hydrothermally treated with CaCl 2 solutions at 200 C for 24 h (TieCaCl 2 ). Two concentrations of CaCl 2 solution were used (TieCaCl 2 10 mM/L and TieCaCl
more » ... 2 20 mM/L). Titanium specimens without CaCl 2 treatment were used as a control. Then, specimens were exposed to L-929 fibroblasts to test cellular attachment and proliferation after 30 min, 1 h, 2 h, 5 h, 1 day, 3 day and 1 week with and without fetal bovine serum (FBS). The percentage of viable attached cells (cells/ml) for each surface treatment was measured using trypan blue assay. Cellular growth on just culture plate material was used as a negative control. The data (n ¼ 5) were statistically analyzed by ANOVA/Tukey test (p < 0.05). Results: Without FBS after 1 week, a statistically significant higher cell attachment to titanium control than to TieCaCl 2 10 mM/L or TieCaCl 2 20 mM/L was revealed. With FBS after 1 week, there was a significant lower cell attachment to TieCaCl 2 20 mM/L than to titanium control. Conclusions: The CaCl 2 hydrothermally treated titanium showed lower evidence of fibroblasts attachment than pure titanium did, which means decreasing the liability of fibrosis, and hence, stronger bonding of CaCl 2 hydrothermally treated titanium implant to bone (better osseointegration).
doi:10.1016/j.tdj.2014.11.004 fatcat:q7k4nekdufhrlcubx4lyzzwsoy