Relative Ability to form Biofilms in Vitro of Staphylococcus Strains Isolated at Implant-Associated Infection and Inflammatory Complications Following Reconstructive Plastic Surgeries
Vestnik of Experimental and Clinical Surgery
Backgraund. The representatives of genus Staphylococcus assign a leading role in the etiology of implant-associated infection, its pathogenesis being closely related to Staphylococcus ability to form biofilms on the implant surfaces. These films suppress the humoral and cellular immunity factors reducing the efficiency of antibiotic treatment and contributing to the infection chronicity. Aims. To study the ability of Staphylococcus spp. strains isolated at infectious complications following the
... tions following the replacement of large joints and reconstructive plastic surgeries in traumatology and orthopedics to form films in vitro. Materials and methods. The ability to form biofilms of 72 Staphylococcus spp. clinical strains was studied in vitro. These strains were isolated from various biological materials of patients with infectious complications following large joint replacements and reconstructive plastic surgeries on the bones of limbs, and Staphylococcus reference strains. The ability to form biofilms was assessed using G.D.Christensen method that implies defining of biomass accumulation and quantitative detection by determining the optical density of gentian violet alcohol extracts in polystyrene microtitre plates. The obtained results were statistically processed using the Statistica 10.0 software. The nonparametric research methods were employed for median (Me) as well as the 25th and 75th quartiles (Q) calculation. The non-parametric Kruskal-Wallis test was used to compare the three samples. The differences were considered significant at p <0.05. Results and their discussion. Staphylococcus strains isolated from biological material of patients with implant-associated infection had statistically (p <0.001) more pronounced ability to form biofilms in vitro, and the ability to form biofilms of S.aureus strains isolated at implant-associated infection was statistically higher (p <0.001) than that of coagulase negative staphylococci. The Staphylococcus spp strains isolated from biological materials of patients with infectious complications following the reconstructive plastic surgeries had statistically lower ability to form biofilms (p <0.001), and no significant interspecies differences were observed in this group. Conclusions. The study of infections and inflammatory agents' ability to form films in traumatology and orthopedics is an essential prerequisite for microbiological diagnostics optimization and improvement of causal treatment efficiency.