In Vitro Phytochemical and Antibacterial Studies on Rhinacanthus Nasutus (L.) Kurz - A Medicinally Important Plant
Journal of Microbiology & Experimentation
The present study was aimed to evaluate and compare the phytochemical and anti-bacterial potential of mother plants (aerial parts), in vitro leaves and stems derived callus and in vitro callus mediated shootlets of Rhinacanthus nasutus (L.) Kurz. Preliminary phytochemical analysis and extraction was performed on stem and leaves segments derived calli (4 weeks old), aerial portions of mother plants and calli mediated shootlets of R. naustus. Anti-bacterial activity of different extracts (25 µg)
... t extracts (25 µg) (ethanol, chloroform, aqueous and ethyl acetate) of stem and leaves segments derived calli, aerial portions of mother plants and calli mediated shootlets were investigated by well-diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. The preliminary phytochemical studies confirmed that the leaves and stem derived calli mediated shootlets showed the higher degree of metabolite constituents and extraction value compared to the in vitro derived calli and in vivo leaves and stem (aerial portions). The ethyl acetate extracted solvents showed the maximum bio-efficacy compared with other solvents due to the presence of more compounds such as saponins, steroids, tannins, phenolics, triterpenoids, alkaloids, coumarins, anthraquinones, glycosides and flavonoids. The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. The methods developed in this work make possible for the low volume and high potential production of active principles under in vitro condition in short duration with less amount of explants utilization. .