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Identification of Live Toxoplasma gondii by the NASBA method in Rat
2012
Modares Journal of Medical Sciences: Pathobiology
unpublished
Objective: Toxoplasmosis is a worldwide disease, for which different detection methods have been used. The nucleic acid sequence-based amplification (NASBA) method is shown to be highly efficient for diagnosis of live microorganisms. The present research evaluates the molecular isothermal method of NASBA to identify live Toxoplasma gondii (T. gondii) in rat. Methods: Tachyzoites of T. gondii were inoculated in the peritoneal cavities of mice (Mus musculus) and their RNA was extracted. The NASBA
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