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Multimerization- and glycosylation-dependent receptor binding of SARS-CoV-2 spike proteins
[article]
2020
bioRxiv
pre-print
Receptor binding studies using recombinant SARS-CoV proteins have been hampered due to challenges in approaches creating spike protein or domains thereof, that recapitulate receptor binding properties of native viruses. We hypothesized that trimeric RBD proteins would be suitable candidates to study receptor binding properties of SARS-CoV-1 and -2. Here we created monomeric and trimeric fluorescent RBD proteins, derived from adherent HEK293T, as well as in GnTI mutant cells, to analyze the
doi:10.1101/2020.09.04.282558
fatcat:aavtprwlcvbufpjgzgdqfdc3ya