Estradiol induces C-type natriuretic peptide gene expression in mouse uterus

Cory G. Acuff, Huaming Huang, Mark E. Steinhelper
1997 American Journal of Physiology. Heart and Circulatory Physiology  
helper. Estradiol induces C-type natriuretic peptide gene expression in mouse uterus. Am. J. Physiol. 273 (Heart Circ. Physiol. 42): H2672-H2677, 1997 have demonstrated that C-type natriuretic peptide (CNP) expression in the uterus varies during the estrous cycle with maximal expression at proestrus. The present study was designed to determine whether exogenous steroid hormones regulate uterine CNP expression in ovariectomized mice. Estradiol increased significantly (3-fold) uterine
more » ... uterine immunoreactive CNP (irCNP) rapidly and dose dependently in ovariectomized mice as measured by radioimmunoassay. Other steroids produced either no significant change (testosterone, 1 mg; 2-methoxyestradiol, 1 µg) or weak induction (estriol, 1 µg) from vehicle controls. Progesterone (1 mg) significantly attenuated the estrogen-stimulated irCNP response by 50% when injected 30 min before estrogen (1 µg) in estrogenprimed ovariectomized mice. Estrogen-stimulated increases in uterine CNP transcripts detected by ribonuclease protection analyses were blocked by actinomycin D (160 µg) or ICI-164,384 (20 µg), a specific nuclear estrogen receptor antagonist. These results indicate that a nuclear estrogen receptor is required for estrogen to stimulate uterine CNP transcription and that progesterone negatively regulates estrogen-stimulated CNP expression. hydromineral homeostasis; reproduction; hyperemia; steroid hormones; estrous cycle MATERIALS AND METHODS
doi:10.1152/ajpheart.1997.273.6.h2672 fatcat:ctzkhcplsjhmjekhuec5ntpiqi