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To characterize the genetic variation between virulent and avirulent isolates of Ralstonia solanacearum race 3 (biovar II), the causal agent of potato brown rot. Nine isolates of R. solanacearum recovered from the natural habitats (potato tubers, weeds, soil and water) were used. Six virulent and three avirulent isolates were characterized in terms of pathogenicity and molecular level using PCR technique with specific primers targeting three-virulence related genes located on the megaplasmiddoi:10.5897/ajmr11.295 fatcat:lheaaudezvbazdajatknm4kdla