Sublingual Immunotherapy Induces Regulatory Function of CD4+CD25+ T cells of CLN in Murine Allergic Rhinitis Model

Kaoru GODA, Takaya YAMADA, Miki TONGU, Takafumi FUCHIWAKI, Chiaki SANO, Hideyuki KAWAUCHI
2010 Nihon Bika Gakkai Kaishi (Japanese Journal of Rhinology)  
Sublingual immunotherapy SLIT has been considered to be a painless and efficacious therapeutic treatment for allergic rhinitis which is known as type I allergy of nasal mucosa in a large number of clinical and basic trials. Putative immunological mechanisms of SLIT are induction of neutralizing antibodies with decrease in IgE/IgG4 ratio and/or induction of antigen specific regulatory T cells Tregs , however those remain controversial. Indeed, the amount of allergen needed in SLIT is 50 to 100
more » ... SLIT is 50 to 100 times more than that in subcutaneous desensitization. In this study, we constructed an efficient murine model of allergic rhinitis with sublingual immunotherapy, in which mice were sublingually administered with ovalbumin OVA followed by intraperitoneal sensitization and nasal challenge of OVA. Sublingually treated mice showed significantly decreased allergic responses as well as suppressed Th2 immune responses. Sublingual administration of OVA did not alter the number of CD4 CD25 Tregs, but lead to up regulation of Foxp3 and IL 10 specific mRNAs in these Tregs of cervical lymph node CLN , which strongly suppressed Th2 cytokine production from CD4 CD25 effector T cells in vitro. These results suggest that Tregs in CLN are involved in the suppression of allergic responses in mice sublingually treated with antigen.
doi:10.7248/jjrhi.49.90 fatcat:5hn6tzs53bhx3dwgiyqzwnfvta