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13C metabolic flux ratio analysis by direct measurement of free metabolic intermediates in L. mexicana using gas chromatography-mass spectrometry
[article]
2016
bioRxiv
pre-print
Until recently, 13C-based flux analyses have almost exclusively relied on analysis of labelled amino acids in proteins. This approach is not directly applicable to Leishmania, as these parasites scavenge most of their amino acids from the media. Leishmania are also unusual in that they i) share little genomic similarity with other organisms ii) constitutively express their metabolic genes and iii) display minimal changes in the enzyme levels throughout their life cycle stages. The three factors
doi:10.1101/073676
fatcat:ipxbp7qnwrdy7ifre7tzpjz6u4