Natronobacterium texcoconense sp. nov., a haloalkaliphilic archaeon isolated from soil of a former lake

E. Ruiz-Romero, K. B. Sanchez-Lopez, M. d. l. A. Coutino-Coutino, S. Gonzalez-Pozos, J. M. Bello-Lopez, M. P. Lopez-Ramirez, D. A. Ramirez-Villanueva, L. Dendooven
2013 International Journal of Systematic and Evolutionary Microbiology  
A novel haloalkaliphilic archaeon, strain B23 T was isolated from the former lake Texcoco in Mexico. The strain was Gram-stain-negative, the cells coccoid to ovoid rods, red pigmented and aerobic. Strain B23 T grew in 1.7-4.3 M NaCl, at pH 6.5-9.5 and at 25-45 6C with optimal growth at 2.6-3.4 M NaCl, pH 7.5-8.5 and 37 6C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B23 T was most closely related to Natronobacterium gregoryi SP2 T with 97.3 % sequence similarity.
more » ... The polar lipids of strain B23 T were phosphatidylglycerol and several unidentified phospholipids. The G+C content of the DNA of the strain was 62.5 mol%. Levels of DNA-DNA relatedness between strain B23 T and Natronobacterium gregoryi DSM 3393 T was 32.3 %. The name Natronobacterium texcoconense sp. nov. is proposed. The type strain is B23 T (5CECT 8068 T 5JCM 17655 T ). Abbreviations: DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen; EC, electrolytic conductivity. The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of strain B23 T is JN571120.
doi:10.1099/ijs.0.053629-0 pmid:23749283 fatcat:njfbczhw3fcnjegj45lnhf76ua