Saffron (Crocus sativus) [book]

M Kafi
‫از‬ ‫برخی‬ ‫بررسی‬ ‫و‬ ‫شناسایی‬ ‫ویژگی‬ ‫های‬ ‫ویروس‬ ‫آلوده‬ ‫کنند‬ ‫ة‬ ‫زعفران‬ ( Crocus sativus ) ‫ایران‬ ‫در‬ ‫پریزاد‬ ‫شیرین‬ 1 ‫دیزجی‬ ‫اکبر‬ ، 2 * ‫حبیبی‬ ‫کوهی‬ ‫مینا‬ ، 2 ، ‫غالمحسین‬ ‫مصاحبی‬ ‫محمدی‬ 3 ‫کالنتری‬ ‫سیامک‬ ، 4 ‫ایزدپناه‬ ‫فاطمه‬ ، 5 ، ‫آرنال‬ ‫گارسیا‬ ‫فرناندو‬ 6 ‫وینتر‬ ‫اشتفان‬ ‫و‬ 7 1 ، 2 ‫و‬ 3 ‫دانشجوی‬ . ‫دکتری،‬ ‫دا‬ ‫و‬ ‫نشیار‬ ‫گروه‬ ‫استاد‬ ‫گ‬ ‫ی‬ ‫اه‬ ‫پزشک‬ ‫ی‬ ، ‫پردیس‬ ‫کشاورزی‬ ‫و‬ ‫منابع‬ ‫طبیعی‬ ‫دانشگاه‬ ‫تهران،‬ ‫کرج‬ 4 ‫و‬ 5 . ‫پردیس‬ ‫باغبانی،‬
more » ... ه‬ ‫سابق‬ ‫دانشجوی‬ ‫و‬ ‫دانشیار‬ ‫کشاورزی‬ ‫و‬ ‫منابع‬ ‫طبیعی‬ ‫دانشگاه‬ ‫تهران،‬ ‫کرج‬ 6 ‫اسپانیا‬ ‫مادرید،‬ ‫مادرید،‬ ‫تکنیک‬ ‫پلی‬ ‫دانشگاه‬ ‫گیاهان،‬ ‫ژنومیکس‬ ‫و‬ ‫بیوتکنولوژی‬ ‫مرکز‬ . 7 ‫کلک‬ . ‫ارگانیسم‬ ‫میکرو‬ ‫آلمانی‬ ‫سیون‬ ‫سلولی‬ ‫کشت‬ ‫و‬ ‫ها‬ DSMZ ‫آلمان‬ ‫برانشوایگ،‬ ، ‫دریافت:‬ ‫(تاریخ‬ 28 / 6 / 1395 -‫پذیرش:‬ ‫تاریخ‬ 23 / 9 / 1395 ) ‫چکیده‬ ‫از‬ ‫پس‬ ‫نمونه‬ ‫بردار‬ ‫از‬ ‫تصادفی‬ ‫ی‬ ‫برگ‬ ‫ها‬ ‫و‬ ‫بنه‬ ‫ها‬ ‫زعفران‬ ‫گیاه‬ ‫ی‬ (Crocus sativus L.) ‫در‬ ‫کشور‬ ‫استان‬ ‫شش‬ ‫از‬ ‫سال‬ ‫ها‬ ‫ی‬ 1390 ‫تا‬ 1394 ‫شمار‬ ، ABSTRACT Following the random sampling of saffron (Crocus sativus L.) leaves and corms from six provinces of Iran, during growing seasons of 2011-2015, a total of 890 leaf tissue samples were investigated by enzyme-linked immunosorbent assay (ELISA) tests. The results revealed that 641 samples showed positive reaction only with Potyvirus genus specific and exactly with Bean common mosaic virus (BCMV) specific antibodies. The molecular mass of several isolates of the virus coat protein (CP) was estimated as 35 kDa by western blotting. Following total RNA extraction from virus infected samples, three discontinuous fragments (corresponding to genomic 3', partial CI and HC-Pro regions) were amplified. The CP region of the virus infecting saffron has the highest nucleotide identity with Ceratobium mosaic virus (CerMV) and Telosma mosaic virus (TelMV) (74%) and the highest deduced amino acid identity with Bean common mosaic necrosis virus (BCMNV) (78.8%). Despite the high serological relationship with BCMV and a close phylogenetic relationship with BCMV subgroup based on CP region, the identity values are less than described species demarcation criteria for Potyviridae family. Overall, it seems that the saffron infecting Potyvirus is a new species of Potyvirus genus but the definitive statement requires sequencing the entire genome of the virus.
doi:10.1201/9781482280463 fatcat:2ef57qpnsfan5p3jymsagn5o5y