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Selection of Linkers for a Catalytic Single-chain Antibody Using Phage Display Technology
Journal of Biological Chemistry
Phage display has been evaluated as a means of rapidly selecting tailored linkers for single-chain antibodies (scFvs) from protein linker libraries. Preliminary experiments with a conventional linker failed to yield a functional single-chain version of a catalytic antibody with chorismate mutase activity. A random linker library was therefore constructed in which the genes for the heavy and light chain variable domains were linked by a segment encoding an 18-amino acid polypeptide of variabledoi:10.1074/jbc.271.26.15682 pmid:8663068 fatcat:r5ldlq6zibhvdp6iomu4ng2hhq