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Myelin formation in rotation-mediated aggregating cell cultures: immunocytochemical, electron microscopic, and biochemical observations
Journal of Neuroscience
Myelination was studied in aggregating cell cultures derived from mechanically dissociated 15 to 16-day fetal rat brains. Myelin basic protein (MBP) and myelin-associated glycoprotein (MAG) were localized immunocytochemically in 20-c-thick Vibratome and l-pm-thick Epon sections at 15, 20, 25, and 30 days in uitro. The occurrence of these proteins was correlated with the ultrastructural appearance of oligodendrocytes and myelin sheaths and with biochemical levels of MBP, MAG, and thedoi:10.1523/jneurosci.02-07-00986.1982 pmid:7097323 fatcat:ujx6c3nyt5cdzgqywnypt7chwq