Biological properties of African swine fever virus Odintsovo 02/14 isolate and its genome analysis

Elsukova, Shevchenko, Varentsova, Puzankova, Zhukov, Pershin, Remyga, Zinyakov, Mazloum, Vlasov, Igolkin, D Lozovoy (+2 others)
2017 International Journal of Environmental & Agriculture Research (IJOEAR)   unpublished
We performed analysis of the biological properties of African swine fever virus (ASF) isolate Odintsovo 02/14. Domestic pigs were inoculated with 50 (low) or 5000 (high) hemadsorbing doses (HAD) of the virus via intranasal (IN) or intramuscular (IM) routes, to investigate the pathogenesis of ASF virus Odintsovo 02/14 isolate. Our results indicated that filtered 10% spleen suspension of ASFV isolate Odintsovo 02/14 induced an acute disease in pigs, resulting in 100% mortality rate. For cultural
more » ... iral suspension (3rd passage), produced in a PBM cells mortality rate was 85.7%. We also present an analysis of the complete genome of African swine fever virus (ASF) Odintsovo 02/14 isolate. It is 189 333 nucleotide long and contains more than 160 open reading frames (ORFs).