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Influence of hyperpnea on airway surface fluid volume and osmolarity in normal humans. To determine the effect of hyperpnea on the characteristics of periciliary liquid, we collected airway surface fluid (ASF) and measured its osmolarity in 11 normal people while they breathed dry, frigid air (Ϫ17 Ϯ 1.2°C) at minute ventilations (V E) of 10, 40, and 80 l/min through a heat exchanger. The ASF was collected at the fifth tracheal ring by absorption onto filter paper pledgets inserted viadoi:10.1152/japplphysiol.00830.2001 pmid:12070199 fatcat:7s7yv263afcr7kelwvmcqmgpca