Lineage and Spatial Mapping of Glioblastoma-associated Immunity [article]

Vidhya Madapusi Ravi, Nicolas Neidert, Paulina Will, Kevin Joseph, Jan Kuekelhaus, Lea Vollmer, Jonathan Goeldner, Simon Behringer, Florian Scherer, Melanie Boerries, Marie Follo, Tobias Weiss (+13 others)
2020 bioRxiv   pre-print
The diversity of molecular states and cellular plasticity of immune cells within the glioblastoma (GBM) environment remain poorly investigated. Here, we performed deep transcriptional profiling of lymphoid and myeloid cell populations by scRNA-sequencing, and mapped potential cellular interactions and cytokine responses that lead to the dysfunctional and exhausted phenotype of T cells. We identified the Interleukin 10 (IL-10) response during T cell activation as leading to a dysfunctional state
more » ... dysfunctional state in T cells. By the use of a novel method, the nearest functionally connected neighbor (NFCN), an in-silico model to explore cell-cell interactions, the dysfunctional/exhausted phenotype was found to be driven by a subset of myeloid cells defined by high expression of HMOX1. By using spatial transcriptomic RNA-sequencing, we identified a correlation between T cell exhaustion and colocalized mesenchymal gene expression also found that HMOX1 expressing myeloid cells occupied regions marked by T cell exhaustion. Using a human neocortical slice model with myeloid cell depletion we confirmed the functional interaction of myeloid and lymphoid cells, leading to the dysfunctional state of T cells. A comprehensive understanding of cellular states and plasticity of lymphoid cells in GBM aids in providing successful immunotherapeutic approaches.
doi:10.1101/2020.06.01.121467 fatcat:q45xqdnoa5gglougojdttp6dei