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Ebony, a Novel Nonribosomal Peptide Synthetase for β-Alanine Conjugation with Biogenic Amines inDrosophila
2003
Journal of Biological Chemistry
Using Ebony protein either expressed in Escherichia coli or in Schneider S2 cells, we provide evidence for its substrate specificity and reaction mechanism. Ebony activates -alanine to aminoacyladenylate by an adenylation domain and covalently attaches it as a thioester to a thiolation domain in a nonribosomal peptide synthetase (NRPS) related mechanism. In a second reaction, biogenic amines act as external nucleophiles on -alanyl-S-pantetheine-Ebony, thereby releasing in a fast reaction the
doi:10.1074/jbc.m304303200
pmid:12900414
fatcat:aqzpkmrbibhwlnodoy7bnhnlv4