Long non-coding RNA XIST promotes cervical cancer cell epithelial-mesenchymal transition through the Wnt/β-catenin pathway

Guixia Sun, Chen Wang, Hongxia Zhang
2017 Int J Clin Exp Pathol   unpublished
Increasing evidences indicated that long non-coding RNAs (lncRNAs) play critical roles in tumor development and progression. However, the role of lncRNA XIST in cervical cancer remains unclear. Methods: QRT-PCR was used to explore the relative expression of XIST in cervical cancer. The biological functions of XIST on cervical cancer cell growth and mobility were determined by CCK-8 assay, transwell migration assay and transwell invasion assay. In addition, the potential mechanism of XIST in
more » ... nism of XIST in cervical cancer progression was investigated by western blot. Results: Our data showed that lncRNA XIST expression was upregulated in cervical cancer tissues and cell lines compared to normal cervical epithelium tissues and normal human cervical epithelial cell line H8. High XIST expression was significantly correlated with cervical cancer lymph node metastasis. Reduced expression of XIST suppressed cervical cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) by modulating Wnt/β-catenin signaling pathway. Conclusion: Our results revealed that lncRNA XIST could act as a novel molecule involved in cervical cancer progression, which provided a potential therapeutic target for new therapies in patients with cervical cancer.