Efficacy of different instrumentation techniques on reducing Enterococcus faecalis infection in experimentally infected root canals

Ebru Özsezer Demiryürek, Elif Kalyoncuoğlu, Esra Duran, Ahmet Yılmaz Çoban, Yeliz Tanrıverdi Çaycı
2014 Journal of Dental Sciences  
Background/purpose: Enterococcus faecalis is the bacterium most commonly isolated from infected root canals. This study evaluated the effects of four instrumentation techniques on reducing E. faecalis in curved root canals. Materials and methods: Sixty maxillary first molar teeth were used. After mesiobuccal canals had been instrumented with nickeletitanium (NiTi) K-files up to size 25, the teeth were autoclaved, immersed in a broth inoculated with E. faecalis, and incubated for 48 hours. The
more » ... for 48 hours. The teeth were divided into four experimental groups (n Z 15). The mesial root canals were instrumented using ProTaper, RaCe, and Mtwo rotary instruments, and hand-operated NiTi files. Irrigation was performed using sterile saline solution. For each root canal, three samplings were performed: twice before preparation and once after preparation. After serial dilutions, samples were incubated, and the colony-forming units were counted. Results: All instrumentation techniques reduced E. faecalis infection in curved root canals. However, there were statistically significant differences among the instrumentation techniques regarding the numbers of residual bacteria (P < 0.01). The ProTaper and RaCe NiTi rotary instruments were more effective than Mtwo and hand-operated NiTi instruments. Conclusion: This study indicates that instruments with a greater taper play an important role in maximizing the effectiveness of mechanical preparation. However, since using mechanical
doi:10.1016/j.jds.2012.03.024 fatcat:ofj4lkaeyzdhvb3uk2lpyyzrtm