Rabbit Normal Regenerating Pancreas Extract induces rat BM-MSCs into Pancreatic Islet-like Structure Cells in vitro
N-RPE induces rat BM-MSCs into ILSs in vitro

Huifeng Wang, Yichen Xu, Liqing Wu, Yali Kou, Zhuo Wu, Wenjing Zhao, Yashu Wei, Hongjing Liu, Weiping Chen
2019 Rabbit Normal Regenerating Pancreas Extract induces rat BM-MSCs into Pancreatic Islet-like Structure Cells in vitro  
Bone marrow mesenchymal stem cells (BM-MSCs) could differentiate into Insulin Producing Cells(IPCs) with notable advantages. The present study tried to develop a method may be able to use a normal regenerating pancreas extract(N-RPE) medium to induce BM-MSCs into islet phenotype, in tests to assess how efficient method and simple duplicate the novel condition medium protocol is. Isolate and purify MSCs from rat bone marrow. BM-MSCs were differentiated into Adipogenic, Osteogenic and Myocardium
more » ... nd the lineages were assessed its multi-lineage potential. Islet differentiation medium, blending rabbit conditioned medium N-RPE, was administered to rat BM-MSCs. After 15 days, differentiation was evaluated by lineage-specific morphology and three stages could be observed: induced cells, islet like cells(ILCs) and islet like structures(ILSs). The morphology, SEM, DTZ staining, Mallory staining, HE staining and glucose stimulation demonstrated that the N-RPE could stimulate suitable development microenvironment to supply the islet differentiate from BM-MSCs. In addition, islet-related genes (ins/glu) expression and proteins(insulin/glucagon) expression suggested that culture medium rabbit N-RPE enhanced the rat BM-MSCs transdifferentiation efficiency. N-RPE derived from normal pancreas tissue could promote pancreas development of microenvironment and significantly enhance the transdifferentiation of BM-MSCs into ILSs. Results from this work will contribute to optimize the conditions of BM-MSCs and supply a new strategy for the development of islet tissue engineering.
doi:10.52274/ispmed20190908 fatcat:tsb2cp6gxvecfigd2brasbpfva