摘要:以人浓缩白细胞来源的 CD14 + 单核细胞为前体,建立体外快速培养树突状细胞(dendritic cell, DC)的 方法。采用密度梯度离心和 MACS 磁珠分选系统,收集高纯度的 CD14 + 单核细胞;以 rGM-CSF、rIL-4 联合分化 2 天诱导不成熟 DC,再将分化后的细胞以 rTNF-α、IL-1β、IL-6、PGE 2 共同活化 2 天得到成熟 DC。流式细胞仪 检测结果表明,分化 2 天的不成熟 DC 具有吞噬能力,且表型 HLA-DR、CD40、CD80 表达在 80%以上,CD83、 CD86 基本不表达,成熟后的 DC 能够激活 T 细胞增殖,HLA-DR 表达增高,CD83、CD86 表达占 85%。 关键词:单核细胞; 树突状细胞;"2+2"快速法 中图分类号:Q25 文献标识码:A Abstract: A "2+2 days" fast protocol for the generation of dendritic cells (DC) from high purity human monocytes in vitro has been

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... tablished. During the 2-step differentiation and activation process, we demonstrated that 2 days of culture with GM-CSF and IL-4 were sufficient to generate immature DCs capable of antigen uptake. Similarly the mature DCs were activated with a cocktail of rTNF-α, IL-1β, IL-6, and PGE 2 from immature DC in 2 days. This "2+2" fast DC had the same phenotype and function as the "6+2" standard DC.