Vitamin C Antioxidant Effect on Gamma – Glutamyl Transferase (Ggt) Activity in Rats under Paraquat Toxicity

Okolonkwo, Benjamin Nnamdi
2012 IOSR Journal of Pharmacy and Biological Sciences  
Paraquat (PQ), a known toxic xenobiotic, has been shown to affect the liver adversely. Its effect on cell membrane integrity has led to the release of membrane enzymes to the extracellular fluid resulting to the increased activity of such enzymes, such as Gamma Glutamyl Transferase (GGT). This study, therefore, has demonstrated that the increased GGT activity as a result of PQ toxicity was reduced as a result of subsequent administration of vitamin C to the animals so treated. The dose and time
more » ... . The dose and time dependent PQ induced toxicity was found to be significantly lower on test subgroups (B 2 , C 2 and D 2 ) when compared to the test subgroups on PQ insult only (B 1 , C 1 and D 1 ). There were health improvement in the antioxidant treated subgroups, such as feeding habit improved, mortality rate reduced, mobility activity increased, eye colour improved and weightgain increase, when compared to the PQ only treated subgroups. The enzyme activity values of the test groups (B, C and D) were highest at month 3 than months 1 and 2 and, higher at month 2 than month 1, indicating that the increased PQ insult duration resulted to more cellular disruption and increased GGT activity. The control group (A) GGT activity was negligibly altered, maintaining values that were at par even up to the end of the research (Month 3). These findings has demonstrated two things, first is that vitamin C is non-toxic to the animals used and second is that the health index used (GGT) was better appreciated in values of vitamin C treated test subgroups than in the subgroups that were on PQ insult only. It is therefore suggested that vitamin C should be included as supplement, in high-enough dose and duration, in the management of patients under PQ toxicity.
doi:10.9790/3008-0140609 fatcat:rminiiprm5hgnlw6lbo6b4ojou