Reactive Oxygen Species Over Production and Superoxide Dismutase Exhaustion Promoting the Intervertebral Disc Degeneration
Biomedical Journal of Scientific & Technical Research
ARTICLE INFO abstract Background: Oxidative overstress is realized as one of the most prevalent causes of Intervertebral Disc Degeneration (IVDD) by restraining the metabolism and damaging the cell matrix, but the specific process is not yet well known. To explore the specific relationship of Reactive Oxygen Species (ROS) and Superoxide Dismutase (SOD) with IVDD. Methods: ICR mice were managed with progressive loading of Upstanding Time (UST) to prepare serial levels of IVDD mouse models. ROS
... d SOD, as the Oxidative Stress (OS) indexes of the endplate and Intervertebral Disc (IVD) from upright mechanical stress, were longitudinally assessed by immunohistochemistry. The endplate cells and structures were synchronously dynamically assayed by histopathology. Results: SOD content in the complexs of the endplate and IVD rapidly increased within 1 to 3 days in the earlier stage after upstanding mechanical stress experiment. Conversely, the ROS level was inhibited until the day 3 after upstanding. Then, SOD decreased gradually, and ROS increased reversely with UST lasting. The histopathology showed that the endplate cartilage cells well-arranged and homogeneous with clear endplate boundaries in the control and 1-day upstanding groups, and the endplate cells swollen until the day 3 after upstanding. With UST lasting, the endplate cells became lysis and sparse, and various structures showed disruption, collapse and blurred in the 9-day group. Conclusion: Oxidative stress is closely related with IVDD, ROS over production and SOD compensatory depletion activating the IVDD.