Aedes aegypti is a major vector of Dengue, a deadly disease causing death of millions of people in developing countries both in urban and rural populations. Ae. aegypti control using chemical insecticide was always done and lead to a widespread insecticide resistance. So, mosquito biological control was needed to replace the usage of chemical insecticide. An experimental study using completely Randomized Design was conducted during March-April 2016 at Balai Litbang P2B2 Banjarnegara. A

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... ide formula was made from Beauveria bassiana secondary metabolite which propagated by The Bacteriology Laboratory of Balai Litbang P2B2 Banjarnegara. In the experimental method, four concentrations of biolarvacide formula (2%, 4%, 8% and16%) were exposed to 3 rd instar of Ae.aegypti larvae for eight days. The result showed that exposure of biolarvacide formula causing larval mortality which started on the 1 st day exposure. This result showed that secondary metabolite of B. bassiana contains some larvacidal compounds.