Aim: The aim of this study is to compare the sealing ability of three different obturation systems using a glucose penetration method. Materials and Methods: A total of 60 extracted mandibular premolar with single root. The samples were decoronated. All the samples were instrumented and obturated with three different obturation techniques i.e. Cold lateral compaction, GuttaFlow2 and Thermafil. After 1 week, the sealing ability of the obturated root canals were tested at different time intervals

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... using glucose penetration test. A spectrophotometer was used to measure glucose leakage. Statistical analysis was done using Kruskal Wallis test. Results: The three groups showed significantly different glucose leakage values at different time intervals (P < 0.05). The glucose leakage was maximum for Group I (Cold Lateral Condensation) as compared to Group II and Group III (GuttaFlow2 and Thermafil). Conclusion: Thermafil system of obturation provided the superior sealing ability over GuttaFlow2 and lateral compaction technique.