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We established the adventitious-root cultures of Iris germanica, a monocotyledonous plant known for isoflavonoid production. Irigenin (5,7,3Ј-trihydroxy-6,4Ј,5Ј-trimethoxyisoflavone) and iristectorigenin A (5,7,3Ј-trihydroxy-6,4Ј-dimethoxyisoflavone) along with their 7-O-b-D-glucosides, iridin and iristectorin A, respectively, were found as the major components in adventitious roots in the liquid medium, and the total isoflavone content was about 3.6 mmol per g fresh weight in 3-week-olddoi:10.5511/plantbiotechnology.22.207 fatcat:qv363vsljng7tmff5jsdninlhu