Solubilization and resolution of thromboxane synthesizing system from microsomes of bovine blood platelets

T Yoshimoto, S Yamamoto, M Okuma, O Hayaishi
1977 Journal of Biological Chemistry  
The thromboxane synthetase system of the microsomes of bovine blood platelets was solubilized by the treatment with Triton X-100. The solubilized preparation was separated into two enzyme fractions by DEAE-cellulose chromatography. One catalyzed the formation of prostaglandin H2 from arachidonic acid in the presence of heme and tryptophan. The other fraction converted prostaglandin H2 to thromboxane B2 and 12L-hydroxy-5,8,10-heptadecatrienoic acid. However, incubation of the latter fraction
more » ... prostaglandin H2 at lower temperature produced an unstable compound with platelet-aggregating activity, which was presumably thromboxane A2 and which decomposed readily to thromboxane B2 and 12L-hydroxy-5,8,10-heptadecatrienoic acid.
pmid:885887 fatcat:bgyaasnf4jd35bc5uqibsh76m4