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Proapoptotic Activity of Cell-Permeable Anti-Akt Single-Chain Antibodies
2005
Cancer Research
We developed anti-Akt1 single-chain antibodies (scFv) by panning a mouse phage-displayed scFv recombinant antibody library. Recombinant scFv that bound glutathione S-transferase (GST)-Akt1 were screened for their ability to inhibit Akt activity in vitro in a kinase reaction containing human recombinant Akt1 and an Akt/serum glucocorticoidinducible kinase (SGK) substrate. Michaelis-Menten analysis of kinase inhibition by a selected scFv was consistent with scFvmediated competition with enzyme's
doi:10.1158/0008-5472.can-04-2898
pmid:15805282
fatcat:vkwyusl7nneqlltoxeakk7i6su