A Simple Method to Test Microscope Objectives Using a Laser Pointer

Thomas Hanscheid
2019 Microscopy Today  
The objective lens is regarded as the critical component for an optimal image in light microscopy. Not every damage to the lens surface may be easily visible during maintenance when the objective is removed and observed. The internal lens organization produces a magnified image of the outer lens surface. Shining the light of a laser pointer into the lens, and reproducing the image of the light exiting onto a flat surface, produces different images for intact and damaged objective lenses.
doi:10.1017/s1551929519000841 fatcat:763mwq3qareivbrwtuom2he2hm