PCR detection of staphylococcal enterotoxin genes and exfoliative toxin genes in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains from raw human breast milk

André Leke, Sabrina Goudjil, Catherine Mullie, Séverine Grognet, Maurice Biendo
2017 Clinical Nutrition Experimental  
Results: S. aureus was identified in 86.2% (62/72) of the 72 Staphylococcus spp isolated from raw milk culture. PCR results showed that 30 of the 62 S. aureus strains (48.3%) harboured genes coding for toxins. sea was the most prevalent virulence gene (24.1%), followed by see (12.9%) and eta (12.9%) genes. Conclusions: Xpert MRSA/SA nasal complete assay allows rapid and accurate identification of MRSA and MSSA. This assay is very easy to perform and is useful for the diagnosis of milk
more » ... s of milk contamination by S. aureus in human milk banks. These results suggest the potential infant health threat related to S. aureus contamination of HBM. Efforts are therefore required to improve safety standards to prevent staphylococcal food poisoning in these infants.
doi:10.1016/j.yclnex.2017.05.001 fatcat:mklooshlwbgt7lpolsknpvklh4