Biomacromolecular Neutron Crystallography

Motoyasu Adachi, Shigeki Arai, Takeshi Hiromoto, Ryota Kuroki
2014 hamon  
Protein structure analysis using neutron diffraction (neutron protein crystallography; NPC) is gaining greater importance in the understanding of structure and function relationships of biological macromolecules such as proteins and DNA. Current developments of neutron diffractometers installed at the JAEA research reactor and pulsed neutron source permit observation of the locations of hydrogen atoms and hydrating water molecules and help understanding of important mechanisms of chemical
more » ... s of chemical reactions catalyzed by biological macromolecules. Here, we introduce practical approaches of NPC including sample preparation, crystal growth, structure determination and utilization of information obtained from NPC.
doi:10.5611/hamon.24.1_45 fatcat:7s5odkth7rcmvibmsxzrrbt7fe