A Novel Signature Based On Mtorc1 Pathway In Hepatocellular Carcinoma [post]

Shijun Zhang, Zhuomao Mo, Shuqiao Zhang
2020 unpublished
BackgroundmTORC1 signal pathway play a role in the initiation and progression of hepatocellular carcinoma (HCC), but no relevant gene signature was developed. This research aimed to explore the potential correlation between mTORC1 signal pathway and HCC and establish the related genes signature.MethodsHCC cases were retrieved from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Expression Omnibus (GEO) databases. The genes to be included in
more » ... in mTORC1-assiociated signature were selected by performing univariate, multivariate Cox regression analysis and lasso regression analysis. Then, the signature was verified by survival analysis and multiple receiver operating characteristic (ROC) curve. Moreover, the correlation between signature and immune cells infiltration was investigated. Furthermore, a nomogram was established and evaluated by C-index and calibration plot.ResultsThe signature was established with the six genes (ETF1, GSR, SKAP2, HSPD1, CACYBP and PNP). Under the grouping from signature, patients in the high- risk group showed worse survival than those in the low-risk group in both three datasets. The signature was found significantly associated with the infiltration of B cells, CD4+T-cells, CD8+T-cells, dendritic cells, macrophages and neutrophils. The univariate and multivariate Cox regression analysis indicated that mTORC1 related signature can be the potential independent prognostic factor in HCC. Finally, the nomogram involved age, gender, stage and signature have been established.ConclusionThe mTORC1 associated gene signature established and validated in our research could be used as a potential prognostic factor in HCC. *These authors contributed equally.
doi:10.21203/rs.3.rs-18272/v1 fatcat:knwaqnxlanaprb7vlcu3ka3gxe