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The TraDIS toolkit: sequencing and analysis for dense transposon mutant libraries
2016
Bioinformatics
Transposon insertion sequencing is a high-throughput technique for assaying large libraries of otherwise isogenic transposon mutants providing insight into gene essentiality, gene function and genetic interactions. We previously developed the Transposon Directed Insertion Sequencing (TraDIS) protocol for this purpose, which utilizes shearing of genomic DNA followed by specific PCR amplification of transposon-containing fragments and Illumina sequencing. Here we describe an optimized high-yield
doi:10.1093/bioinformatics/btw022
pmid:26794317
pmcid:PMC4896371
fatcat:wmejtnbxtjg7vgppqgkhmkubkq