Engineering bacterial strains through the chromosomal insertion of the chlorocatechol catabolism tfdICDEF gene cluster, to improve degradation of typical bleached Kraft pulp mill effluent pollutants

Roberto Bobadilla, Cristián Varela, Ricardo Céspedes, Bernardo González
2002 Electronic Journal of Biotechnology  
Engineering bacterial strains through the chromosomal insertion of the chlorocatechol catabolism tfdI CDEF gene cluster, to improve degradation of typical bleached Kraft pulp mill effluent pollutants Chloroaromatic pollutants from bleached Kraft pulp mill effluents (BKME) are difficult to degrade, because bacterial strains present in BKME aerobic treatments, only partially degrade these compounds, accumulating the corresponding chlorocatechol intermediates. To improve the catabolic performance
more » ... f chlorocatecholaccumulating strains, we introduced, by chromosomal insertion, the tfd I CDEF gene cluster from Ralstonia eutropha JMP134 (pJP4). This gene cluster allows dechlorination and channelling of chlorocatechols into the intermediate metabolism. Two bacterial strains, R. eutropha JMP222 and Pseudomonas putida KT2442, able to produce chlorocatechols from 3-chlorobenzoate (3-CB) were used. Acinetobacter lwoffii RB2 isolated from BKME by its ability to grow on guaiacol as sole carbon source and shown to be able to produce the corresponding chlorocatechols from the BKME pollutants 4-, and 5-chloroguaiacol, was also used. The HERRERO, Marta; DE LORENZO, Victor and TIMMIS, Kenneth N. Transposon vectors containing non-antibiotic selection markers for cloning and stable chromosomal insertion of foreign DNA in Gram-negative bacteria. DAUBARAS, D.L.; CHAKRABARTY, A.M.; KINZELT, D. and REINEKE, W. Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway.
doi:10.2225/vol5-issue2-fulltext-7 fatcat:k5yfsx2q6vcczemjl2cno72opa